University of Massachusetts Medical School
UMass Flow Cytometry Core Facility

Design a panel and find antibodies today at

https://app.fluorofinder.com/umassmed
Logo: FluoroFinder

C-Aria II Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

 Alexa Fluor 350
CMA-Chromomycin A3
DAPI
Hoechst (33342)
Indo-Hi (Ca2+bound)
Live/Dead Fix Blue
CF350
DyLight 350

670 / 50

635

 Hoechst (33342)
Laser

405

450 / 50

--

 Alexa Fluor 405
Cascade Blue
eCFP
Pacific Blue
Pacific Orange
Brilliant Violet 421
CF405M
DyLight 405
eFluor 450
V450

525 / 50

505

 Alexa Fluor 430
AmCyan
Cascade Yellow
Live/Dead Fix Aqua
Qdot 525
Brilliant Violet 510
DyLight 405LS
Krome Orange
Pacific Orange
V500

605 / 40

595

 Alexa Fluor 430
Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Pacific Orange
Qdot 605
SuperBright 600
Laser

488

525 / 50

505

 Alexa Fluor 488
CFSE
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488

582 / 14

550

 dsRed
PE

610 / 20

600

 ECD
EMA
PE-Texas Red
Propidium Iodide
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

710 / 50

685

 PE-Cy5
PE-Cy5.5
PerCP-Cy5.5
PerCP-eFluor 710
7-AAD
Brilliant Blue 700
PerCP-Vio700

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

561

582 / 14

--

 Cy3
dsRed
mRFP1
PE
tdTomato
Alexa Fluor 546
Alexa Fluor 555
CF550R
CF568
CF570

610 / 20

600

 Alexa Fluor 568
Alexa Fluor 594
Cy3.5
mCherry
mStrawberry
PE-Texas Red
Propidium Iodide
Texas Red
BODIPY TR
CF583

660 / 20

635

 7-AAD

710 / 50

685

 PE-Cy5
PE-Cy5.5

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

633

670 / 30

--

 Alexa Fluor 633
Alexa Fluor 635
Alexa Fluor 647
APC
Cy5
CF640R
CF647
Cyanine 5 SE
DyLight 633
DyLight 650

730 / 44

690

 Alexa Fluor 700
APC-Alexa 700
APC-Cy5.5
eFluor 710
Alexa Fluor 680
APC-R700
DyLight 680

780 / 60

750

 Alexa Fluor 750
APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.