University of Massachusetts Medical School
UMass Flow Cytometry Core Facility

Design a panel and find antibodies today at

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LSR IIB-3 Daisy Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Alexa Fluor 405
Cascade Blue
eCFP
eFluor 450
Live/Dead Fix Violet
Pacific Blue
Pacific Orange
V450
Vio Blue
Brilliant Violet 421

525 / 20

475

 Alexa Fluor 430
AmCyan
Cascade Yellow
Live/Dead Fix Aqua
Qdot 525
Vio Green
Brilliant Violet 510
DyLight 405LS
Krome Orange
Pacific Green

605 / 20

600

 Brilliant Violet 605
Qdot 605
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600

660 / 30

630

 Brilliant Violet 650
Qdot 655
eFluor 650NC
eVolve 655
SuperBright 645

800 / 80

760

 Brilliant Violet 785
Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

505

 Alexa Fluor 488
CFSE
eGFP
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

575 / 26

556

 dsRed
PE

670 / 14

635

 PE-Cy5
PE-Cy5.5
PerCP
PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700
7-AAD

780 / 60

735

 PE-Cy7
PE-Vio770
PE-Alexa 750
Laser

633

660 / 20

--

 Alexa Fluor 633
Alexa Fluor 635
Alexa Fluor 647
APC
Cy5
CF640R
CF647
Cyanine 5 SE
DyLight 633
DyLight 650

730 / 44

685

 Alexa Fluor 700
APC-Alexa 700
APC-Cy5.5
eFluor 710
Alexa Fluor 680
APC-R700
DyLight 680

780 / 60

755

 Alexa Fluor 750
APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
APC-Vio770
APC-Fire 750

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.