University of Massachusetts Medical School
UMass Flow Cytometry Core Facility

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LSR IIA-5 Donald Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

375 / 28

--

 Brilliant Ultraviolet 395

450 / 50

420

 Alexa Fluor 350
CMA-Chromomycin A3
DAPI
Hoechst (33342)
Indo-Hi (Ca2+bound)
Live/Dead Fix Blue
CF350
DyLight 350

525 / 20

505

 Indo-Lo (Ca2+free)
Live/Dead Fix Aqua
Laser

405

450 / 50

--

 Alexa Fluor 405
Cascade Blue
eCFP
eFluor 450
Live/Dead Fix Violet
Pacific Blue
Pacific Orange
V450
Brilliant Violet 421
CF405M

525 / 20

475

 Alexa Fluor 430
AmCyan
Cascade Yellow
Live/Dead Fix Aqua
Qdot 525
V500
Brilliant Violet 510
DyLight 405LS
Krome Orange
Pacific Orange

605 / 20

600

 Brilliant Violet 605
Qdot 605
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600

660 / 32

630

 Brilliant Violet 650
Qdot 655
eFluor 650NC
eVolve 655
SuperBright 645

800 / 80

760

 Brilliant Violet 785
Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

505

 Alexa Fluor 488
CFSE
eGFP
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

695 / 40

685

 PE-Cy5
PE-Cy5.5
PerCP-Cy5.5
PerCP-eFluor 710
7-AAD
Brilliant Blue 700
PerCP-Vio700
Laser

561

580 / 20

570

 Alexa Fluor 546
dsRed
mRFP1
PE
tdTomato
Alexa Fluor 555
ATTO 550
CF550R
CF568
CF570

610 / 20

600

 Alexa Fluor 568
Alexa Fluor 594
Cy3.5
mCherry
mStrawberry
PE-Texas Red
Propidium Iodide
Texas Red
BODIPY TR
CF583

660 / 20

635

 PE-Cy5
Propidium Iodide
7-AAD

710 / 50

685

 PE-Cy5.5
Propidium Iodide

780 / 60

755

 PE-Cy7
Propidium Iodide
PE-Vio770
Laser

633

660 / 20

--

 Alexa Fluor 633
Alexa Fluor 635
Alexa Fluor 647
APC
Cy5
CF640R
CF647
Cyanine 5 SE
DyLight 633
DyLight 650

720 / 30

700

 Alexa Fluor 700
APC-Alexa 700
APC-Cy5.5
eFluor 710
Alexa Fluor 680
APC-R700
DyLight 680

785 / 60

755

 Alexa Fluor 700
APC-Alexa 750
APC-Cy7
APC-Fire 750
APC-H7
APC-Vio770
APC-eFluor 780

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.