University of Massachusetts Medical School
UMass Flow Cytometry Core Facility

Design a panel and find antibodies today at

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LSR IIA Uncle Fester Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

 Alexa Fluor 350
CMA-Chromomycin A3
DAPI
Hoechst (33342)
Indo-Hi (Ca2+bound)
Live/Dead Fix Blue
CF350
DyLight 350

530 / 30

505

 Indo-Lo (Ca2+free)
Live/Dead Fix Aqua
Laser

405

450 / 50

--

 Alexa Fluor 405
Cascade Blue
eCFP
Pacific Blue
Pacific Orange
Brilliant Violet 421
CF405M
DyLight 405
eFluor 450
V450

525 / 50

505

 Alexa Fluor 430
AmCyan
Cascade Yellow
Live/Dead Fix Aqua
Qdot 525
Brilliant Violet 510
DyLight 405LS
Krome Orange
Pacific Orange
V500
Laser

488

530 / 30

505

 Alexa Fluor 488
CFSE
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488

575 / 26

555

 dsRed
PE
Propidium Iodide

695 / 40

680

 PE-Cy5
PE-Cy5.5
PerCP-Cy5.5
7-AAD
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

800 / 64

735

 PE-Cy7
PE-Vio770
Laser

633

660 / 20

--

 Alexa Fluor 633
Alexa Fluor 635
Alexa Fluor 647
APC
Cy5
CF640R
CF647
Cyanine 5 SE
DyLight 633
DyLight 650

800 / 64

735

 Alexa Fluor 750
APC-Alexa 750
APC-Cy7
APC-H7
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.