University of Massachusetts Amherst
Veterinary and Animal Sciences Flow Cytometry Core Facility

Design a panel and find antibodies today at

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Amnis ImageStream Configuration

Laser (nm) BP SP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

--

505

430

 Alexa Fluor 405
DAPI
Pacific Blue
Brilliant Violet 421
CF405M
DyLight 405
eFluor 450
V450
Vio Blue

--

570

505

 Alexa Fluor 430
Pacific Orange
Qdot 525
Brilliant Violet 510
Brilliant Violet 570
DyLight 405LS
Krome Orange
V500
Vio Green

--

640

595

 eFluor 625NC
Qdot 625
Brilliant Violet 605

702 / 85

--

--

 Brilliant Violet 650
Brilliant Violet 711
eFluor 650NC
Qdot 705
eVolve 655
Qdot 655
SuperBright 645
SuperBright 702

762 / 35

--

--

 Brilliant Violet 785
Qdot 800
Brilliant Violet 786
Laser

488

533 / 55

--

--

 Alexa Fluor 488
DyLight 488
FITC
GFP
LysoTracker Green
MitoTracker Green
PKH67
Spectrum Green
YFP
ATTO 488

577 / 35

--

--

 Cy3
dsRed
mOrange
PE
PKH26
Sytox Orange
Alexa Fluor 555
Cyanine 3 SE

610 / 30

--

--

 ECD
eFluor 625NC
PE-Alexa 610
PE-Texas Red
Qdot 625
RFP
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

702 / 85

--

--

 DRAQ 5
eFluor 650NC
PE-Alexa 647
PE-Cy5
PerCP
PerCP-Cy5.5
Propidium Iodide
Qdot 705
7-AAD
Brilliant Blue 700

762 / 35

--

--

 PE-Alexa 700
Qdot 800
Laser

642

702 / 85

--

--

 Alexa Fluor 647
Alexa Fluor 660
Alexa Fluor 680
APC
Cy5
DRAQ 5
DyLight 649
DyLight 680
PE-Alexa 647
PE-Cy5
PerCP
PerCP-Cy5.5

762 / 35

--

--

 Alexa Fluor 750
APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
Cy7
DyLight 750
PE-Alexa 750
PE-Cy7
APC-Fire 750

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.