University of Massachusetts Amherst
Veterinary and Animal Sciences Flow Cytometry Core Facility

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LSR Fortessa (5 laser, Right Side) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

 Brilliant Ultraviolet 395

450 / 50

410

 DAPI
Indo-Hi (Ca2+bound)
DyLight 350

740 / 35

690

 Brilliant Ultraviolet 737
Laser

405

450 / 50

--

 Brilliant Violet 421
Pacific Blue
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
V450
Vio Blue

525 / 50

505

 Brilliant Violet 510
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
V500
Vio Green

610 / 20

595

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

710 / 50

695

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

750

 Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

505

 Alexa Fluor 488
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

710 / 50

685

 PE-Cy5.5
PerCP
PerCP-Cy5.5
7-AAD
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

585 / 15

--

 PE
CF550R
CF568
CF570

610 / 20

600

 dsRed
PE-Texas Red
Propidium Iodide
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610

670 / 30

635

 PE-Cy5

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

 APC
Alexa Fluor 647
CF640R
CF647
Cy5
DyLight 633
NL 637

730 / 45

685

 Alexa Fluor 700
APC-R700

780 / 60

750

 APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.