University of California, San Francisco (UCSF)
Parnassus Flow Cytometry Core

Design a panel and find antibodies today at

https://app.fluorofinder.com/ucsf-parnassus
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HSE301D Stay Puft Marshmallow Man Fortessa X50 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

 Brilliant Ultraviolet 395
Indo-Hi (Ca2+bound)

515 / 30

495

 Brilliant Ultraviolet 496
Indo-Lo (Ca2+free)

586 / 15

570

 Brilliant Ultraviolet 563

610 / 20

600

 Qdot 605
eVolve 605

660 / 20

640

 Brilliant Ultraviolet 661

740 / 35

690

 Brilliant Ultraviolet 737

820 / 60

770

 Brilliant Ultraviolet 805
Laser

405

450 / 50

--

 Brilliant Violet 421
DAPI
Pacific Blue
SuperBright 436
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
V450
Vio Blue

470 / 15

450

 Brilliant Violet 480

515 / 20

505

 Brilliant Violet 510
Alexa Fluor 430
CF405L
CF430
CF440
V500
Vio Green

586 / 15

570

 Brilliant Violet 570
DyLight 405LS
Qdot 585

610 / 20

595

 Brilliant Violet 605
SuperBright 600
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605

660 / 20

640

 Brilliant Violet 650
SuperBright 645
eFluor 650NC
eVolve 655
Qdot 655

710 / 50

685

 Brilliant Violet 711
SuperBright 702
Qdot 705

750 / 30

735

 Brilliant Violet 750

800 / 30

770

 Brilliant Violet 785
Brilliant Violet 786
Qdot 800
Laser

488

515 / 20

505

 Alexa Fluor 488
Brilliant Blue 515
CFSE
FITC
Sytox Green
ATTO 488
CF488A
CF503R
Cy2
DyLight 488

660 / 20

635

 PerCP

710 / 50

685

 Brilliant Blue 700
PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700

780 / 60

750

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

561

586 / 15

--

 Cy3
dsRed
dTomato
PE
RFP
Alexa Fluor 546
Alexa Fluor 555
CF550R
CF568
CF570

610 / 20

595

 Alexa Fluor 594
mCherry
PE-CF594
PE-Dazzle 594
PE-Texas Red
Propidium Iodide
BODIPY TR
CF583
CF583R
DyLight 594

660 / 40

640

 PE-Cy5
7-AAD

710 / 50

690

 PE-Cy5.5

780 / 60

750

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

628

660 / 20

--

 Alexa Fluor 633
Alexa Fluor 647
APC
Cy5
eFluor 660
Sytox Red
Alexa Fluor 635
CF640R
Cyanine 5 SE
DyLight 633

730 / 45

690

 Alexa Fluor 680
Alexa Fluor 700
APC-Cy5.5
APC-R700
DyLight 680

780 / 60

750

 Alexa Fluor 750
APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.