CD19 Spark PLUS B574 (HIB19, Mouse) - Reactivity: Human

About CD19
This gene encodes a member of the immunoglobulin gene superfamily. Expression of this cell surface protein is restricted to B cell lymphocytes. This protein is a reliable marker for pre-B cells but its expression diminishes during terminal B cell differentiation in antibody secreting plasma cells. The protein has two N-terminal extracellular Ig-like domains separated by a non-Ig-like domain, a hydrophobic transmembrane domain, and a large C-terminal cytoplasmic domain. This protein forms a complex with several membrane proteins including complement receptor type 2 (CD21) and tetraspanin (CD81) and this complex reduces the threshold for antigen-initiated B cell activation. Activation of this B-cell antigen receptor complex activates the phosphatidylinositol 3-kinase signalling pathway and the subsequent release of intracellular stores of calcium ions. This protein is a target of chimeric antigen receptor (CAR) T-cells used in the treatment of lymphoblastic leukemia. Mutations in this gene are associated with the disease common variable immunodeficiency 3 (CVID3) which results in a failure of B-cell differentiation and impaired secretion of immunoglobulins. CVID3 is characterized by hypogammaglobulinemia, an inability to mount an antibody response to antigen, and recurrent bacterial infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020]

About Spark PLUS B574
Spark PLUS B574™ from Biolegend is a fluorophore for use in both conventional and spectral flow cytometry with an excitation peak at 506 nm and an emission peak at 574 nm. While it’s suitable for both conventional and spectral flow, it demonstrates maximum brightness on spectral cytometers. It can be unmixed from colors like Spark PLUS B550™, FITC, and PerCP/Cyanine5.5, which makes it a good option for large spectral panels. It can be unmixed from PE on instruments with both blue and yellow/green lasers.