University of California, Los Angeles (UCLA)
UCLA JCCC Flow Cytometry Core Facility

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ImageStream Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

457 / 45

--

 Brilliant Violet 421
DAPI
GFP
Hoechst (33258)
Live/Dead Fix Violet
Pacific Blue
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450

533 / 55

--

 Brilliant Violet 510
Pacific Orange
Alexa Fluor 430
Brilliant Violet 570
CF405L
CF430
CF440
DyLight 405LS
Krome Orange
V500

577 / 35

--

 eFluor 565NC
Qdot 565
Brilliant Violet 570

610 / 30

--

 Brilliant Violet 605
Qdot 625
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

702 / 85

--

 Brilliant Violet 711
Qdot 705
SuperBright 702

762 / 35

--

 Brilliant Violet 786
Qdot 800
Brilliant Violet 785
Laser

488

533 / 55

--

 Alexa Fluor 488
FITC
GFP
LysoTracker Green
MitoTracker Green
PKH67
YFP
ATTO 488
Brilliant Blue 515
CF488A

577 / 35

--

 Cy3
PE
PKH26
Alexa Fluor 555
Cyanine 3 SE

610 / 30

--

 ECD
PE-Alexa 610
PE-Texas Red
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

702 / 85

--

 DRAQ 5
LDS 751
PE-Alexa 647
PE-Cy5
PerCP
7-AAD
PerCP-Vio700

762 / 35

--

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

642

702 / 85

--

 Alexa Fluor 647
Alexa Fluor 660
APC
Cy5
DRAQ 5
PE-Cy5
PerCP
PerCP-Cy5.5
Alexa Fluor 635
CF640R

762 / 35

--

 APC-Alexa 750
APC-Cy7
APC-H7
Cy7
PE-Cy7
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.