University of California, Los Angeles (UCLA)
UCLA JCCC Flow Cytometry Core Facility

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SORP FACSAria II Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

DAPI
Hoechst (33258)
Indo-Hi (Ca2+bound)
DyLight 350

525 / 50

505

Indo-Lo (Ca2+free)
Laser

488

525 / 50

505

Alexa Fluor 488
Brilliant Blue 515
FITC
GFP
ATTO 488
Cy2
DyLight 488
Fluorescein
NL 493

695 / 40

670

PerCP
PerCP-Cy5.5
7-AAD
Laser

561

582 / 15

--

PE
Propidium Iodide

610 / 20

600

dsRed
mCherry
mStrawberry
PE-Texas Red
DyLight 594

780 / 60

750

PE-Cy7
Laser

640

670 / 30

--

Alexa Fluor 647
APC
Cy5
DyLight 633
DyLight 650
NL 637

710 / 50

690

Alexa Fluor 680
Alexa Fluor 700
APC-Cy5.5
APC-R700
DyLight 680

780 / 60

750

APC-Alexa 750
APC-Cy7

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.