University of California, Los Angeles (UCLA)
UCLA JCCC Flow Cytometry Core Facility

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SORP BD HTS LSR II Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

Alexa Fluor 405
Pacific Blue
Brilliant Violet 421
CF405M
DyLight 405
eFluor 450
V450
Vio Blue

525 / 50

505

Alexa Fluor 430
Pacific Orange
Brilliant Violet 510
DyLight 405LS
Krome Orange
V500
Vio Green

605 / 40

595

Qdot 605
Brilliant Violet 605
DyLight 405LS
eVolve 605

660 / 20

630

Qdot 655
Brilliant Violet 650
eVolve 655
Laser

488

525 / 50

502

Alexa Fluor 488
Brilliant Blue 515
FITC
GFP
ATTO 488
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

585 / 42

550

dsRed
PE
Propidium Iodide

610 / 20

600

PE-Texas Red
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

710 / 50

685

PerCP-Cy5.5
7-AAD
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

780 / 60

735

PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

710 / 50

690

Alexa Fluor 680
Alexa Fluor 700
APC-Cy5.5
APC-R700
DyLight 680

780 / 60

755

APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.