University of California, Los Angeles (UCLA)
UCLA JCCC Flow Cytometry Core Facility

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SORP LSRFortessa X-20 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
CFP
Pacific Blue
CF405M
DyLight 405
eFluor 450
SuperBright 436
V450
Vio Blue

525 / 50

495

 Alexa Fluor 430
AmCyan
Brilliant Violet 510
Pacific Orange
Qdot 525
CF405L
CF430
CF440
DyLight 405LS
Krome Orange

610 / 20

595

 Brilliant Violet 605
eFluor 605NC
Qdot 605
DyLight 405LS
eVolve 605
SuperBright 600

670 / 30

635

 Brilliant Violet 650
eFluor 650NC
Qdot 655
eVolve 655
SuperBright 645

710 / 50

690

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

750

 Brilliant Violet 786
Qdot 800
Brilliant Violet 785
Laser

488

530 / 30

505

 Alexa Fluor 488
Brilliant Blue 515
FITC
ATTO 488
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
NL 493

586 / 15

550

 PE

610 / 20

595

 PE-Texas Red
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

695 / 40

690

 PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

710 / 50

685

 Alexa Fluor 680
Alexa Fluor 700
APC-Alexa 700
APC-Cy5.5
APC-R700
DyLight 680

780 / 60

755

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.