University of Chicago
Flow Cytometry Facility (UC)

Design a panel and find antibodies today at

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FACSAria Fusion 5-18 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

Brilliant Ultraviolet 395

515 / 30

450

Brilliant Ultraviolet 496

820 / 60

770

Brilliant Ultraviolet 805
Laser

405

450 / 50

--

DAPI

525 / 50

505

AmCyan
Alexa Fluor 430
Brilliant Violet 510
V500
Vio Green

610 / 20

550

Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

670 / 30

635

Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 50

695

Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

750

Brilliant Violet 785
Qdot 800
Laser

488

515 / 20

495

FITC
Alexa Fluor 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
Vio Bright FITC

710 / 50

685

PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

585 / 15

--

PE
CF550R
CF568
CF570

610 / 20

595

PE-Texas Red
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

670 / 30

635

PE-Cy5

780 / 60

750

PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

APC
Alexa Fluor 647
CF640R
CF647
Cy5
DyLight 633
NL 637

730 / 45

695

Alexa Fluor 700
APC-R700

780 / 60

750

APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.