University of Connecticut
UConn Health Flow Cytometry Core

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ZE5 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

387 / 11

--

 Brilliant Ultraviolet 395

447 / 60

--

 Alexa Fluor 350
Brilliant Ultraviolet 395
CF350

525 / 50

--

 Brilliant Ultraviolet 496

670 / 30

--

 Brilliant Ultraviolet 661

--

700

 Brilliant Ultraviolet 737
Brilliant Ultraviolet 805
Laser

405

420 / 10

--

 Brilliant Violet 421
Alexa Fluor 405
DyLight 405
SuperBright 436
Vio Blue

460 / 22

--

 Brilliant Violet 480

525 / 50

--

 Brilliant Violet 510
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
V500
Vio Green

615 / 24

--

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

670 / 30

--

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

720 / 60

--

 Brilliant Violet 711
Qdot 705
SuperBright 702

--

750

 Brilliant Violet 750
Brilliant Violet 785
Brilliant Violet 786
Qdot 800
Laser

488

525 / 35

--

 Alexa Fluor 488
CFSE
FITC
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

593 / 52

--

 PE-CF594
ECD
PE-Dazzle 594
PE-Texas Red
PE-Vio615

692 / 80

--

 PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

--

750

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

561

577 / 15

--

 PE
CF550R
CF568
CF570

589 / 15

--

 PE-Dazzle 594
PE-DyLight 594

615 / 24

--

 PE-Texas Red
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

640 / 20

--

 PE-Fire 640

670 / 30

--

 PE-Alexa 647
PE-Cy5
PE-Cy5.5

720 / 60

--

 PE-Alexa 700
PE-Fire 700

--

750

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

 APC
Alexa Fluor 647
CF640R
CF647
Cy5
DyLight 633
NL 637

720 / 60

--

 APC-R700
Alexa Fluor 700

775 / 50

--

 APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

--

800

 APC-Fire 810

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.