University of Connecticut
UConn Health Flow Cytometry Core

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LSR II-B Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

 Alexa Fluor 350
DAPI
Hoechst (33342)
Indo-Hi (Ca2+bound)
Live/Dead Fix Blue
CF350
DyLight 350

525 / 50

505

 Brilliant Ultraviolet 496
Indo-Lo (Ca2+free)

660 / 20

630

 Brilliant Ultraviolet 661

710 / 50

685

 Brilliant Ultraviolet 737
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
CFP
eFluor 450
Live/Dead Fix Violet
Pacific Blue
CF405M
DyLight 405
SuperBright 436
V450

525 / 50

505

 Alexa Fluor 430
Brilliant Violet 510
eFluor 506 Fix Viability
Pacific Orange
V500
CF405L
CF430
CF440
DyLight 405LS
Krome Orange

585 / 42

570

 Brilliant Violet 570
Qdot 585
DyLight 405LS
Qdot 565

605 / 40

595

 Brilliant Violet 605
eFluor 605NC
Qdot 605
DyLight 405LS
eVolve 605
SuperBright 600

660 / 40

630

 Brilliant Violet 650
eFluor 650NC
Qdot 655
eVolve 655
SuperBright 645

710 / 50

670

 Brilliant Violet 711
Qdot 705
SuperBright 702
Laser

488

530 / 30

505

 Alexa Fluor 488
CFSE
FITC
GFP
YFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

695 / 40

635

 PerCP
PerCP-Cy5.5
PerCP-eFluor 710
Propidium Iodide
7-AAD
Brilliant Blue 700
PerCP-Vio700
Laser

561

582 / 14

--

 dsRed
PE
RFP
Rhodamine
tdTomato
CF550R
CF568
CF570
TRITC

610 / 20

600

 Alexa Fluor 594
mCherry
PE-CF594
PE-Texas Red
Propidium Iodide
BODIPY TR
CF583
CF583R
DyLight 594
ECD

780 / 60

750

 PE-Cy7
PE-Vio770
PE-Alexa 750
Laser

640

670 / 30

--

 Alexa Fluor 647
APC
Cy5
Live/Dead Fix Far Red
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cyanine 5 SE
DyLight 633

720 / 30

685

 Alexa Fluor 700
APC-Cy5.5
Alexa Fluor 680
APC-R700
DyLight 680

780 / 60

755

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Vio770
Live/Dead Fix Near IR (775)
APC-Fire 750
APC-H7

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.