Temple University
School of Medicine Flow Cytometry Core Facility

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Influx Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

407

460 / 50

--

 Brilliant Violet 421
eFluor 450
eFluor 450 Fix Viability
Live/Dead Fix Violet
Pacific Blue
Sytox Blue
V450
Vybrant DyeCycle Violet
Alexa Fluor 405
CF405M

520 / 35

480

 AmCyan
Brilliant Violet 510
CFP
Live/Dead Fix Aqua
V500
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS

605 / 40

575

 Brilliant Violet 605
eVolve 605
Qdot 605
DyLight 405LS
eFluor 605NC
SuperBright 600

--

650

 Brilliant Violet 650
eVolve 655
Qdot 655
eFluor 650NC
SuperBright 645
Laser

488

530 / 40

--

 Alexa Fluor 488
Brilliant Blue 515
CFSE
eFluor 520 Fix Viability
FITC
GFP
Live/Dead Fix Green
Sytox Green
Vybrant DyeCycle Green
YFP

692 / 40

600

 PE-Cy5
PE-Cy5.5
PE-Cy7
PerCP
PerCP-Cy5.5
PerCP-eFluor 710
Propidium Iodide
Sytox AADvanced
7-AAD
Brilliant Blue 700
Laser

561

585 / 29

--

 dsRed
mOrange
PE
CF550R
CF568
CF570

610 / 20

600

 mCherry
mRuby
PE-Texas Red
Propidium Iodide
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594

660 / 20

645

 mKate2
mPlum
PE-CF594
PE-Cy5
7-AAD
DyLight 594

692 / 40

685

 PE-Cy5.5

--

750

 PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

 Alexa Fluor 647
APC
Cy5
eFluor 660
eFluor 660 Fix Viability
Sytox Red
TO-Pro 3
Alexa Fluor 633
Alexa Fluor 635
CF640R

720 / 40

700

 Alexa Fluor 700
DRAQ 5
APC-R700

--

750

 APC-Cy7
APC-eFluor 780
APC-H7
eFluor 780 Fix Viability
APC-Alexa 750
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.