Temple University
School of Medicine Flow Cytometry Core Facility

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LSR II Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

 Brilliant Ultraviolet 395

450 / 50

450

 Alexa Fluor 350
DAPI
DyLight 350
eFluor 455UV Fix Viability
Hoechst (33258)
Live/Dead Fix Blue
Vybrant DyeCycle Violet
CF350

740 / 35

690

 Brilliant Ultraviolet 737
Laser

405

450 / 50

--

 Pacific Blue
V450
Alexa Fluor 405
Brilliant Violet 421
CF405M
DyLight 405
eFluor 450
Vio Blue

525 / 50

505

 AmCyan
Brilliant Violet 510
V500
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
Vio Green

605 / 40

600

 Brilliant Violet 605
Qdot 605
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600

660 / 20

630

 Brilliant Violet 650
Qdot 655
eFluor 650NC
eVolve 655
SuperBright 645

780 / 60

750

 Brilliant Violet 750
Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

505

 Alexa Fluor 488
Brilliant Blue 515
CFSE
Cy2
DyLight 488
eFluor 520 Fix Viability
FITC
Fluorescein
GFP
Live/Dead Fix Green
Sytox Green
Vio Bright FITC
Vybrant DyeCycle Green
YFP

575 / 26

550

 PE
RFP

695 / 40

685

 PE-Cy5
PE-Cy5.5
PerCP
PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700
Propidium Iodide
Sytox AADvanced
7-AAD
Brilliant Blue 700

780 / 60

735

 PE-Cy7
PE-Vio770
PE-Alexa 750
Laser

640

670 / 30

--

 Alexa Fluor 647
APC
Cy5
DyLight 633
DyLight 650
eFluor 660 Fix Viability
NL 637
Sytox Red
TO-Pro 3
Alexa Fluor 633

730 / 45

690

 Alexa Fluor 700
APC-R700
DRAQ 5

780 / 60

735

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770
eFluor 780 Fix Viability

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.