SickKids - UHN
Flow Cytometry Facility-PMCRT node

Design a panel and find antibodies today at

https://app.fluorofinder.com/sickkids-pmctr
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Aria Fusion A Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
eBFP
eFluor 450
eFluor 450 Fix Viability
FVS450
Live/Dead Fix Violet
Pacific Blue
SuperBright 436
Sytox Blue
V450
Zombie Violet

525 / 50

505

 Alexa Fluor 430
AmCyan
Brilliant Violet 480
Brilliant Violet 510
eCFP
eFluor 506
eFluor 506 Fix Viability
FVS510
Live/Dead Fix Aqua
Pacific Orange
Qdot 525
V500
Zombie Aqua

610 / 20

600

 Brilliant Violet 605
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600
DyLight 405LS

660 / 20

635

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 50

685

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

750

 Brilliant Violet 785
Brilliant Violet 786
Qdot 800
SuperBright 780
Laser

488

515 / 20

505

 Alexa Fluor 488
Brilliant Blue 515
CellROX Green
CFSE
FITC
FVS520
GFP
Live/Dead Fix Green
Thiazole Orange
YFP
Zombie Green

720 / 40

690

 Brilliant Blue 700
PerCP
PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700
PE-Cy5.5
Laser

532

575 / 26

--

 Alexa Fluor 546
Alexa Fluor 555
CellROX Orange
Cy3
dsRed
eFluor 570
FVS570
PE
RFP
Sytox Orange
tdTomato

610 / 20

600

 FVS620
Live/Dead Fix Red
mCherry
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Texas Red
Propidium Iodide
Zombie Red
ECD

660 / 20

635

 mKate2
PE-Cy5
Propidium Iodide
Sytox AADvanced
7-AAD

720 / 40

685

 PE-Alexa 700
PE-Cy5.5

780 / 60

755

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

640

660 / 20

--

 Alexa Fluor 633
Alexa Fluor 647
APC
CellROX Deep Red
Cy5
eFluor 660
eFluor 660 Fix Viability
FVS660
Live/Dead Fix Far Red
Sytox Red

710 / 50

685

 Alexa Fluor 700
APC-R700
FVS700

780 / 60

755

 Alexa Fluor 750
APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770
eFluor 780 Fix Viability
FVS780
Live/Dead Fix Near IR (775)
Zombie NIR

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.