SickKids - UHN
Flow Cytometry Facility-PGCRL node

Design a panel and find antibodies today at

https://app.fluorofinder.com/sickkids-pgcrl
Logo: FluoroFinder

Symphony A3 B (UVBYR) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

 Brilliant Ultraviolet 395

515 / 30

450

 Brilliant Ultraviolet 496

586 / 15

550

 Brilliant Ultraviolet 563

610 / 20

600

 Brilliant Ultraviolet 615

670 / 30

635

 Brilliant Ultraviolet 661

740 / 35

690

 Brilliant Ultraviolet 737

820 / 60

770

 Brilliant Ultraviolet 805
Laser

409

431 / 28

410

 Brilliant Violet 421

470 / 24

450

 Brilliant Violet 480

586 / 15

550

 Brilliant Violet 570

610 / 20

600

 Brilliant Violet 605

670 / 20

635

 Brilliant Violet 650

710 / 50

690

 Brilliant Violet 711

740 / 35

710

 Brilliant Violet 750

780 / 60

750

 Brilliant Violet 786
Laser

488

515 / 30

505

 Brilliant Blue 515
FITC
Alexa Fluor 488
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
Vio Bright FITC

610 / 20

600

 Brilliant Blue 630-P2

670 / 30

635

 Brilliant Blue 660-P2

710 / 50

690

 PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

780 / 60

770

 Brilliant Blue 790-P
Laser

561

586 / 15

--

 PE
CF550R
CF568
CF570

610 / 20

600

 PE-CF594
DyLight 594
ECD
PE-Dazzle 594
PE-Texas Red
PE-Vio615

670 / 20

650

 PE-Cy5

710 / 50

690

 PE-Alexa 700

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

637

670 / 30

650

 Alexa Fluor 633
Alexa Fluor 647
APC
CF640R
CF647
Cy5
DyLight 633
NL 637
Alexa Fluor 635
CF633

730 / 45

690

 Alexa Fluor 700
APC-R700

780 / 60

750

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.