SickKids - UHN
Flow Cytometry Facility-PGCRL node

Design a panel and find antibodies today at

https://app.fluorofinder.com/sickkids-pgcrl
Logo: FluoroFinder

LSR II GC (VBR) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

407

431 / 28

--

 Brilliant Violet 421
Pacific Blue
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
V450
Vio Blue

610 / 20

600

 Brilliant Violet 605
Qdot 625
StarBright Violet 610
SuperBright 600
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605

780 / 60

755

 Brilliant Violet 785
Brilliant Violet 786
Qdot 800
Laser

488

515 / 30

505

 Alexa 488 NanoGold
Alexa Fluor 488
ATTO 488
Brilliant Blue 515
CYTO-ID Green
DyLight 488
eYFP
FITC
GFP
Vio Bright FITC
Vybrant DyeCycle Green

575 / 26

550

 PE

610 / 20

600

 FVS620
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Texas Red
Propidium Iodide
ECD
PE-Vio615

660 / 20

635

 PE-Cy5
PerCP
PerCP-Cy5
Propidium Iodide
Sytox AADvanced
7-AAD
PerCP-Vio700

710 / 50

685

 PE-Alexa 700
PE-Cy5.5
PerCP-Vio700

780 / 60

755

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

633

660 / 20

--

 Alexa Fluor 633
Alexa Fluor 647
APC
CellROX Deep Red
Cy5
eFluor 660
eFluor 660 Fix Viability
FVS660
Live/Dead Fix Far Red
Sytox Red

720 / 40

685

 Alexa Fluor 700
APC-Cy5.5
APC-R700
ATTO 655
CellVue Plum
Vybrant DyeCycle Ruby
Alexa Fluor 680
DyLight 680

780 / 60

755

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770
eFluor 780 Fix Viability
FVS780
Live/Dead Fix Near IR (780)
Zombie NIR

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.