SickKids - UHN
Flow Cytometry Facility-PGCRL node

Design a panel and find antibodies today at

https://app.fluorofinder.com/sickkids-pgcrl
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Sony MA900 (BRYV) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

Alexa Fluor 405
Brilliant Violet 421
DAPI
eBFP
eFluor 450
eFluor 450 Fix Viability
FVS450
Live/Dead Fix Violet
Pacific Blue
SuperBright 436
Sytox Blue
V450
Zombie Violet

525 / 50

--

Alexa Fluor 430
AmCyan
Brilliant Violet 480
Brilliant Violet 510
eCFP
eFluor 506
eFluor 506 Fix Viability
FVS510
Live/Dead Fix Aqua
Qdot 525
V500
Zombie Aqua

585 / 30

--

Brilliant Violet 570
Pacific Orange
Qdot 585
Zombie Yellow
Alexa Fluor 430
DyLight 405LS
Krome Orange
Qdot 565

617 / 30

--

Brilliant Violet 605
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600
DyLight 405LS

665 / 30

--

Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

720 / 60

--

Brilliant Violet 711
Qdot 705
SuperBright 702

785 / 60

--

Brilliant Violet 785
Brilliant Violet 786
Qdot 800
SuperBright 780
Laser

488

525 / 50

--

Alexa Fluor 488
Brilliant Blue 515
CellROX Green
CFSE
FITC
FVS520
GFP
Live/Dead Fix Green
Thiazole Orange
YFP
Zombie Green

585 / 30

--

PE

617 / 30

--

FVS620
PE-CF594
PE-Dazzle 594
PE-eF610
PE-Texas Red
Propidium Iodide
ECD
PE-eFluor 610
PE-Vio615

695 / 40

--

Brilliant Blue 700
PerCP
PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700
PE-Cy5
PE-Cy5.5

785 / 60

--

PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

561

585 / 30

--

Alexa Fluor 546
Alexa Fluor 555
CellROX Orange
Cy3
dsRed
eFluor 570
FVS570
PE
RFP
Sytox Orange
tdTomato

617 / 30

--

Alexa Fluor 594
FVS620
Live/Dead Fix Red
mCherry
mKate2
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Texas Red
Propidium Iodide
Zombie Red

695 / 50

--

mPlum
PE-Alexa 700
PE-Cy5
PE-Cy5.5
Sytox AADvanced
7-AAD

785 / 60

--

PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

638

665 / 30

--

Alexa Fluor 633
Alexa Fluor 647
APC
CellROX Deep Red
Cy5
eFluor 660
eFluor 660 Fix Viability
FVS660
Live/Dead Fix Far Red
Sytox Red

720 / 60

--

Alexa Fluor 700
APC-R700
FVS700

785 / 60

--

Alexa Fluor 750
APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770
eFluor 780 Fix Viability
FVS780
Live/Dead Fix Near IR (775)
Zombie NIR

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.