University of Florida
The Wertheim UF Scripps Institute

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Aria III Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
DAPI
Pacific Blue
CF405M
DyLight 405
eFluor 450
SuperBright 436
V450
Vio Blue

525 / 50

502

 Brilliant Violet 510
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
V500
Vio Green

585 / 42

550

 Pacific Orange
Alexa Fluor 430
Brilliant Violet 510
Brilliant Violet 570
DyLight 405LS
Krome Orange

610 / 20

600

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

670 / 30

635

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 50

685

 Brilliant Violet 711
Qdot 705
SuperBright 702
Laser

488

530 / 30

502

 Alexa Fluor 488
CFSE
FITC
GFP
YFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

695 / 40

655

 PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

582 / 15

--

 dsRed
PE
RFP
tdTomato
CF550R
CF568
CF570

610 / 20

600

 Alexa Fluor 594
mCherry
PE-Texas Red
Propidium Iodide
BODIPY TR
CF583
CF583R
DyLight 594
ECD
PE-CF594

670 / 14

630

 PE-Cy5

710 / 50

685

 PE-Cy5.5

780 / 60

755

 PE-Cy7
PE-Vio770
Laser

633

660 / 20

--

 Alexa Fluor 647
APC
Cy5
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cyanine 5 SE
DyLight 633
DyLight 650

730 / 45

675

 Alexa Fluor 700
APC-Cy5.5
Alexa Fluor 680
APC-R700
DyLight 680

780 / 60

755

 Alexa Fluor 750
APC-Alexa 750
APC-Cy7
APC-Fire 750
APC-H7
APC-Vio770
APC-eFluor 780

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.