Scripps Research Institute La Jolla (TSRI)
Scripps Flow Cytometry Core Facility

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LSR (B) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

405 / 20

--

Indo-Hi (Ca2+bound)

530 / 30

--

Indo-Lo (Ca2+free)
Laser

407

440 / 40

--

Pacific Blue
Alexa Fluor 405
Brilliant Violet 421
CF405M
DyLight 405
eFluor 450
V450
Vio Blue

525 / 50

--

AmCyan
Alexa Fluor 430
Brilliant Violet 510
V500
Vio Green
Laser

488

530 / 30

--

FITC
Alexa Fluor 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
Vio Bright FITC

575 / 26

--

PE

695 / 40

--

PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

780 / 60

--

PE-Cy7
PE-Vio770
Laser

640

660 / 20

--

APC
Alexa Fluor 647
CF640R
CF647
Cy5
DyLight 633
NL 637

780 / 60

--

APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.