Scripps Research Institute La Jolla (TSRI)
Scripps Flow Cytometry Core Facility

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Bio-Rad ZE5 5 laser 27 color Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

387 / 11

--

 Brilliant Ultraviolet 395

447 / 60

--

 Alexa Fluor 350
DAPI
Hoechst (33342)
CF350
DyLight 350

525 / 50

--

 Brilliant Ultraviolet 496

670 / 30

--

 Hoechst (33342)

--

700

 Brilliant Ultraviolet 737
Brilliant Ultraviolet 805
Laser

405

420 / 10

--

 Brilliant Violet 421
Alexa Fluor 405
DyLight 405
SuperBright 436
Vio Blue

460 / 22

--

 Pacific Blue
StarBright Violet 440
StarBright Violet 475
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
V450
Vio Blue

525 / 50

505

 Brilliant Violet 510
Live/Dead Fix Aqua
StarBright Violet 475
StarBright Violet 515
Vio Green
Alexa Fluor 430
AmCyan
Cascade Yellow
CF405L
CF430

615 / 24

--

 Brilliant Violet 605
Live/Dead Fix Yellow
StarBright Violet 610
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

670 / 30

--

 Brilliant Violet 650
eVolve 655
StarBright Violet 670
eFluor 650NC
Qdot 655
SuperBright 645

720 / 50

690

 Brilliant Violet 711
StarBright Violet 710
Qdot 705
SuperBright 702

--

750

 Brilliant Violet 785
StarBright Violet 760
StarBright Violet 790
Qdot 800
Laser

488

525 / 35

--

 Alexa Fluor 488
FITC
GFP
YFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488

593 / 52

--

 LSS-mKate1
LSS-mKate2
mHoneyDew
Nile Red

692 / 80

--

 PerCP
PerCP-Cy5.5
StarBright Blue 700
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

--

750

 PE-Cy7
PE-Vio770
Laser

561

577 / 15

--

 PE
Sytox Orange
CF550R
CF568
CF570

589 / 15

--

 dsRed
dTomato
tdTomato

615 / 24

--

 mCherry
PE-CF594
DyLight 594
ECD
PE-Dazzle 594
PE-Texas Red
PE-Vio615

640 / 20

--

 mPlum
mRaspberry

670 / 30

--

 PE-Cy5

720 / 60

--

 PE-Cy5.5

--

750

 PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

720 / 60

--

 Alexa Fluor 700
APC-R700

775 / 50

--

 APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

--

800

 Alexa Fluor 790

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.