Scripps Research Institute La Jolla (TSRI)
Scripps Flow Cytometry Core Facility

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Beckman Coulter CytoFLEX S V4-B2-Y4-R3 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 45

--

 Alexa Fluor 405
Brilliant Violet 421
eFluor 450
V450
CF405M
DyLight 405
Pacific Blue
SuperBright 436
Vio Blue

525 / 40

--

 Brilliant Violet 510
Pacific Green
V500
Alexa Fluor 430
AmCyan
CF405L
CF430
CF440
DY-380XL
DyLight 405LS

610 / 20

--

 Brilliant Violet 605
Qdot 605
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600

660 / 10

--

 Brilliant Violet 650
Qdot 655
eFluor 650NC
eVolve 655
SuperBright 645
Laser

488

525 / 40

--

 Alexa Fluor 488
DyLight 488
Vio Bright 515
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
FITC
Fluorescein

690 / 50

--

 PE-Cy5
PerCP-Cy5.5
7-AAD
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

585 / 42

--

 PE
Propidium Iodide
CF550R
CF568
CF570

610 / 20

--

 PE-Dazzle 594
PE-eFluor 610
PE-Texas Red
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Vio615

690 / 50

--

 PE-Cy5

780 / 60

--

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

638

660 / 10

--

 Alexa Fluor 647
Cy5
Alexa Fluor 633
Alexa Fluor 635
APC
CF640R
CF647
Cyanine 5 SE
NL 637

712 / 25

--

 Alexa Fluor 700
APC-R700

780 / 60

--

 APC-Alexa 750
APC-Cy7
APC-H7
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.