Scripps Research Institute La Jolla (TSRI)
Scripps Flow Cytometry Core Facility

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Agilent NovoCyte 3000 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

445 / 44

--

 Alexa Fluor 405
Brilliant Violet 421
DAPI
eFluor 450
Pacific Blue
Vio Blue
Cascade Blue
CF405M
DyLight 405
eBFP

530 / 30

--

 Brilliant Violet 510
Pacific Orange
Vio Green
Alexa Fluor 430
AmCyan
Cascade Yellow
CF405L
CF430
CF440
DyLight 405LS

585 / 40

--

 Brilliant Violet 570
DyLight 405LS
Qdot 565
Qdot 585

615 / 24

--

 Brilliant Violet 605
eFluor 605NC
eVolve 605
Qdot 605
DyLight 405LS
SuperBright 600

675 / 30

--

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

780 / 60

--

 Brilliant Violet 785
Qdot 800
Laser

488

530 / 30

--

 Alexa Fluor 488
Brilliant Blue 515
FITC
GFP
Vio Bright FITC
ATTO 488
CF488A
CF503R
Cy2
DyLight 488

585 / 40

--

 PE

615 / 24

--

 PE-CF594
PE-Dazzle 594
PE-Texas Red
ECD
PE-eFluor 610
PE-Vio615

675 / 30

--

 PE-Cy5
PE-Cy5.5
PerCP
PerCP-Vio700
PerCP-Cy5.5
PerCP-eFluor 710

780 / 60

--

 PE-Cy7
PE-Vio770
PE-Alexa 750
Laser

640

675 / 30

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

780 / 60

--

 APC-Cy7
APC-Vio770
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.