Sanford Burnham Prebys Medical Discovery Institute
SBP Flow Cytometry Shared Resource

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Fortessa Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Brilliant Violet 421
DAPI
Pacific Blue
V450
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
Vio Blue

525 / 50

475

 AmCyan
Brilliant Violet 510
Qdot 525
V500
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
Vio Green

605 / 12

595

 Brilliant Violet 605
Qdot 605
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600

660 / 20

630

 Brilliant Violet 650
Qdot 655
SuperBright 645
eFluor 650NC
eVolve 655

710 / 50

685

 Brilliant Violet 711
SuperBright 702
Qdot 705
Laser

488

510 / 25

495

 FITC
GFP
Alexa Fluor 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
Vio Bright FITC

695 / 40

685

 PerCP
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

585 / 15

--

 PE
CF550R
CF568
CF570

610 / 20

600

 Alexa Fluor 568
mCherry
PE-Texas Red
Propidium Iodide
CF583
CF583R
Cy3.5
DyLight 594
Dyomics 590
ECD

670 / 30

635

 PE-Cy5

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

640

670 / 14

--

 Alexa Fluor 633
Alexa Fluor 647
APC
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

730 / 45

710

 Alexa Fluor 700
APC-R700

780 / 60

750

 APC-Cy7
APC-H7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.