Sanford Burnham Prebys Medical Discovery Institute
SBP Flow Cytometry Shared Resource

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FACSAria 1 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Brilliant Violet 421
eFluor 450
Pacific Blue
V450
Alexa Fluor 405
CF405M
DyLight 405
SuperBright 436
Vio Blue

530 / 30

505

 AmCyan
Brilliant Violet 510
V500
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
Vio Green

610 / 20

595

 Brilliant Violet 605
eFluor 605NC
Qdot 605
DyLight 405LS
eVolve 605
SuperBright 600

670 / 30

635

 Brilliant Violet 650
eFluor 650NC
Qdot 655
eVolve 655
SuperBright 645

710 / 50

685

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

750

 Brilliant Violet 785
Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

505

 Alexa Fluor 488
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

680 / 37

635

 PerCP
PerCP-Cy5.5
PerCP-eFluor 710
Brilliant Blue 700
PerCP-Vio700
Laser

561

586 / 15

--

 PE
CF550R
CF568
CF570

610 / 20

595

 ECD
mCherry
PE-CF594
PE-eFluor 610
PE-Texas Red
CF583
CF583R
DyLight 594
PE-Dazzle 594
PE-Vio615

670 / 30

630

 PE-Cy5

710 / 50

685

 PE-Alexa 700

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

 Alexa Fluor 647
APC
eFluor 660
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650

730 / 45

690

 Alexa Fluor 700
APC-R700

780 / 60

750

 Alexa Fluor 750
APC-Cy7
APC-eFluor 780
APC-H7
APC-Alexa 750
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.