Penn State University
Huck Institute Microscopy and Cytometry Facility

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Fortessa - BV605 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Alexa Fluor 405
BFP
Brilliant Violet 421
Cascade Blue
CFP
Pacific Blue
CF405M
DyLight 405
eFluor 450
SuperBright 436

525 / 20

505

 AmCyan
Brilliant Violet 510
Qdot 525
V500
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
Vio Green

610 / 20

595

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600
Laser

488

525 / 50

505

 Alexa Fluor 488
CFSE
FITC
GFP
YFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

685 / 35

635

 PerCP
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

532

575 / 25

550

 Alexa Fluor 532
dsRed
PE
ATTO 532
ATTO 532 Phalloidin
CF550R
CF570

610 / 20

595

 Alexa Fluor 568
Alexa Fluor 594
mCherry
PE-Texas Red
Propidium Iodide
RFP
BODIPY TR
ECD
PE-CF594
PE-Dazzle 594

660 / 20

635

 PE-Cy5

695 / 40

685

 PE-Cy5.5

780 / 40

735

 PE-Cy7
PE-Vio770
Laser

640

670 / 30

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 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

710 / 50

685

 Alexa Fluor 700
APC-R700

780 / 60

750

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.