Penn State University
Huck Institute Microscopy and Cytometry Facility

Design a panel and find antibodies today at

https://app.fluorofinder.com/psuhuck
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FlowSight Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

467 / 75

--

 Alexa Fluor 405
Cascade Blue
CFP
DAPI
DyLight 405
eFluor 450
Hoechst (33258)
Live/Dead Fix Violet
Pacific Blue
Spectrum Aqua

532 / 55

--

 Alexa Fluor 430
Cascade Yellow
Lucifer Yellow
Pacific Orange
Qdot 525
Qdot 545
Brilliant Violet 510
Brilliant Violet 570
DyLight 405LS
Krome Orange

577 / 35

--

 Qdot 565
Qdot 585
Brilliant Violet 570

618 / 47

--

 eFluor 605NC
Qdot 605
Qdot 625
Brilliant Violet 605
DyLight 405LS
eVolve 605

691 / 98

--

 eFluor 650NC
Qdot 705
Brilliant Violet 650
eVolve 655
Qdot 655

770 / 60

--

 Qdot 800
Brilliant Violet 785
Brilliant Violet 786
Laser

488

532 / 55

--

 Acridine Orange
Alexa Fluor 488
Alexa Fluor 514
Calcium Green
DyLight 488
FITC
GFP
LysoTracker Green
MitoTracker Green
PKH67
Spectrum Green
Syto 9
Vybrant DyeCycle Green
YFP

577 / 35

--

 DiI
dsRed
PE
PKH26

618 / 47

--

 ECD
PE-Alexa 610
PE-Texas Red
7-AAD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

691 / 98

--

 DRAQ 5
PE-Alexa 647
PE-Alexa 700
PE-Cy5
PE-Cy5.5
PerCP
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

770 / 60

--

 PE-Cy7
PE-Vio770
Laser

642

691 / 98

--

 Alexa Fluor 647
Alexa Fluor 660
Alexa Fluor 680
APC
APC-Cy5.5
APC-R700
Cy5
Cy5.5
DRAQ 5
DyLight 649
MitoTracker Deep Red
Nile Blue

770 / 60

--

 Alexa Fluor 750
APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
DyLight 750
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.