Penn State University
Huck Institute Microscopy and Cytometry Facility

Design a panel and find antibodies today at

https://app.fluorofinder.com/psuhuck
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Astrios Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

448 / 59

--

DAPI
DyLight 350
Hoechst (33258)

620 / 29

--

Propidium Iodide

692 / 75

--

Hoechst (33342)
Laser

405

448 / 59

--

Alexa Fluor 405
Cascade Blue
CFP
DyLight 405
Pacific Blue
Brilliant Violet 421
CF405M
eFluor 450
V450
Vio Blue

546 / 20

--

Cascade Yellow
Pacific Orange
Qdot 565
Alexa Fluor 430
Brilliant Violet 510
Brilliant Violet 570
DyLight 405LS
Krome Orange
V500
Vio Green
Laser

488

513 / 26

--

Alexa Fluor 488
CFSE
FITC
GFP
YFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

576 / 21

--

CMA-Chromomycin A3
PE

620 / 29

--

Alexa Fluor 568
Alexa Fluor 594
ECD
PE-Texas Red
Propidium Iodide
Rhodamine
BODIPY TR
PE-CF594
PE-Dazzle 594
PE-eFluor 610

664 / 22

--

PE-Cy5
PerCP-Vio700

710 / 45

--

PE-Cy5.5
PerCP-Vio700

795 / 70

--

PE-Cy7
PE-Vio770
Laser

532

576 / 21

--

Alexa Fluor 532
dsRed
PE
RFP
tdTomato
ATTO 532
ATTO 532 Phalloidin
CF550R
CF570

622 / 22

--

mCherry
PE-Alexa 610

664 / 22

--

PE-Alexa 647

692 / 18

--

PE-Cy5.5

736 / 47

--

PE-Alexa 700
Laser

640

671 / 30

--

Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

722 / 44

--

Alexa Fluor 700
APC-R700

795 / 70

--

APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.