FluoroFinder

	Supplier	Novus Biologicals, a Bio-Techne brand
	Catalog #:	NBP2-20872 (View supplier product page)
	Size	0.1 ml
	Price	$449.00
	Antigen	VPS33A
	Clone
	Host	Rabbit
	Isotype	IgG
	Conjugate	Unconjugated
	Target Species	Human, Mouse
	Applications	WB, IHC, ICC/IF, IHC-P
	Description	Rabbit VPS33A antibody [Purified] reacts with Human, Mouse

	VPS33A	This gene encodes a tethering protein and a core subunit of the homotypic fusion and protein sorting (HOPS) complex. The HOPS complex and a second endosomal tethering complex called the class C core vacuole/endosome tethering (CORVET) complex, perform diverse functions in endocytosis including membrane tethering, RabGTPase interaction, activation and proofreading of synaptic-soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) assembly to drive membrane fusion, and endosome-to-cytoskeleton attachment. The HOPS complex controls endosome maturation as well as endosome traffic to the lysosome. This complex is essential for vacuolar fusion and is required for adaptor protein complex 3-dependent transport from the golgi to the vacuole. The encoded protein belongs to the Sec1/Munc18 (SM) family of SNARE-mediated membrane fusion regulators. Naturally occurring mutations in this gene are associated with a novel mucopolysaccharidosis-like disease. [provided by RefSeq, Apr 2017]

		Immunocytochemistry/Immunofluorescence: VPS33A Antibody [NBP2-20872] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS33A protein stained by VPS33A antibody [C1C3] diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:1000. Blue: Hoechst 33342 staining.
		Western Blot: VPS33A Antibody [NBP2-20872] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS33A antibody [C1C3] (119416) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
		Immunohistochemistry-Paraffin: VPS33A Antibody [NBP2-20872] - Immunohistochemical analysis of paraffin-embedded colon ca, using antibody at 1:500 dilution.