FluoroFinder

	Supplier	Novus Biologicals, a Bio-Techne brand
	Catalog #:	NBP1-86569 (View supplier product page)
	Size	0.1 ml
	Price	$519.00
	Antigen	MAP3K8
	Clone
	Host	Rabbit
	Isotype	IgG
	Conjugate	Unconjugated
	Target Species	Human
	Applications	WB, IHC, ICC/IF, IHC-P
	Description	Specificity of human MAP3K8/Tpl2/COT antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

	MAP3K8	This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

		Immunocytochemistry/Immunofluorescence: MAP3K8/Tpl2/COT Antibody [NBP1-86569] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
		Immunohistochemistry-Paraffin: MAP3K8/Tpl2/COT Antibody [NBP1-86569] - Staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.
		Western Blot: MAP3K8/Tpl2/COT Antibody [NBP1-86569] - Analysis in control (vector only transfected HEK293T lysate) and MAP3K8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).