FluoroFinder

	Supplier	R&D Systems
	Catalog #:	MAB9578-100 (View supplier product page)
	Size	100 μg
	Price	$429.00
	Antigen	LRP-2
	Clone	545606
	Host	Mouse
	Isotype	IgG1
	Conjugate	Unconjugated
	Target Species	Human
	Applications	FC, IHC, CyTOF
	Description	Human Megalin/LRP2 Antibody

	LRP-2	The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).[provided by RefSeq, Aug 2009]

		Detection of Megalin/LRP2 in CaCo-2 Human Cell Line by Flow Cytometry. CaCo2 human cell line was stained with Mouse Anti-Human Megalin/LRP2 Monoclonal Antibody (Catalog # MAB9578, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
		Megalin/LRP2 in Human Kidney. Megalin/LRP2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Megalin/LRP2 Monoclonal Antibody (Catalog # MAB9578) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.