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Laminin gamma 1 Monoclonal / Unconjugated / CL3195

Product Details

	Supplier	Novus Biologicals, a Bio-Techne brand
	Catalog #:	NBP2-42394 (View supplier product page)
	Size	0.1 ml
	Price	$519.00
	Antigen	Laminin gamma 1
	Clone	CL3195
	Host	Mouse
	Isotype	IgG2b
	Conjugate	Unconjugated
	Target Species	Human
	Applications	WB, IHC, IHC-P
	Description	Specificity of human Laminin gamma 1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

About Laminin gamma 1 and Purified

	Laminin gamma 1	Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis. [provided by RefSeq, Aug 2011]

Images

		Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3195) [NBP2-42394] - Staining of human rectum shows strong immunoreactivity in basement membrane of glandular epithelium.
		Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3195) [NBP2-42394] - Staining of human testis shows strong positivity in basement membrane in seminiferous tubules.
		Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3195) [NBP2-42394] - Staining of human kidney shows strong immunoreactivity in basement membrane of renal tubules and membranous positivity in glomeruli.
		Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3195) [NBP2-42394] - Staining of human cerebellum shows strong immunoreactivity in endothelium.
		Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3195) [NBP2-42394] - Staining of human lymph node shows absence of immunoreactivity in lymphoid cells (negative control).
		Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3195) [NBP2-42394] - Staining of human fallopian tube shows strong positivity in basement membrane of glandular epithelium.
		Western Blot: Laminin gamma 1 Antibody (CL3195) [NBP2-42394] - Lane 1: Marker [kDa] 460, 268, 238, 171, 117, 71, 55, 41, 31 Lane 2: Purified human recombinant protein Laminin-111 Lane 3: Purified human recombinant protein Laminin-121 Lane 4: Purified human recombinant protein Laminin-211 Lane 5: Purified human recombinant protein Laminin-221 Lane 6: Purified human recombinant protein Laminin-411 Lane 7: Purified human recombinant protein Laminin-421 Lane 8: Purified human recombinant protein Laminin-511 Lane 9: Purified human recombinant protein Laminin-521 Lane 10: Purified human recombinant protein Laminin-332

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