Western Blot: KDEL Antibody (2C1) [NBP2-61926] - analysis of Human HeLa cell lysates showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control. Lane 4: Anti-PDI control. Lane 5: Anti-Calreticulin control. Lane 6: Anti-GRP78 control. Lane 7: Anti-GRP94 control. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.