FluoroFinder

	Supplier	Novus Biologicals, a Bio-Techne brand
	Catalog #:	NBP1-32714 (View supplier product page)
	Size	100 ul
	Price	$379.00
	Antigen	GLE1
	Clone
	Host	Rabbit
	Isotype	IgG
	Conjugate	Unconjugated
	Target Species	Human, Mouse
	Applications	WB, IHC, ICC/IF, IHC-P

	GLE1	This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

		Immunocytochemistry/Immunofluorescence: GLE1 Antibody [NBP1-32714] - Paraformaldehyde-fixed HeLa, using GLE1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.
		Western Blot: GLE1 Antibody [NBP1-32714] - Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE, antibody diluted at 1:1000.
		Immunohistochemistry-Paraffin: GLE1 Antibody [NBP1-32714] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.