Immunocytochemistry: Follistatin Antibody ([17-2]) - Azide and BSA Free [NBP2-61941] - Clone 17/2 used to detect FS288 expression in fetal and adult Leydig cells by ICC. Follistatin immunoexpression was first detectable immediately after birth in the fetal generation of Leydig cells (a, day 7, arrows) and immunostaining was equally intense in the adult generation of Leydig cells when they appeared (b, day 27) and remained intense to adulthood (not shown).

Immunohistochemistry: Follistatin Antibody ([17-2]) - Azide and BSA Free [NBP2-61941] - Clone 17/2 used to detect immunoreactivity with FS288 by IHC-P. Immunolocalization of FS315 and FS288 to PC3 and LNCaP cell lines. Positive immunoreactivity for FS315 localized to the cytoplasm of the epithelial tumor cell lines LNCaP (A) and PC3 (B) using H10 antibody, no immunoreactivity was present if the antibody was preabsorbed with FS315 peptide (inset A, inset B). Mouse IgG antibody controls were negative (inset C, inset D).

ELISA: Follistatin Antibody ([17-2]) - Azide and BSA Free [NBP2-61941] - Antibody 17/2 used to detect Fst levels using two-site ELISA with antibody 29/9. Dose-response curves for various human biological fluid samples containing follistatin using the optimized ELISA procedure.

Western Blot: Follistatin Antibody ([17-2]) - Azide and BSA Free [NBP2-61941] - Western Blots showing binding of 17/2 to isoforms of FS288. A. Protein bands corresponding to the approximate 31 and 35 kDa molecular weight isoforms of FS were detected in PC3 media (lane 2) and lanes containing hrFS288 protein.