Immunocytochemistry/Immunofluorescence: FAM8A1 Antibody [NBP2-47125] - Hek293 cells were fixed for 5 minute using 10% formalin and then permeabilized for 10 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-FAM8A1 at a 1:500 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: FAM8A1 Antibody [NBP2-47125] - Western Blot Image of anti-FAM8A1. Total protein from human brain (lane 1), skeletal muscle (lane 2), mouse brain (lane 3) and rat testis (lane 4) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-FAM8A1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry-Paraffin: FAM8A1 Antibody [NBP2-47125] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse testes using FAM8A1 antibody at 1:200 dilution. The antibody generated a cytoplasmic staining with intense brown dots (potentially the staining of the ER) in each of the primary and secondary spermatocytic cells.

Immunohistochemistry-Paraffin: FAM8A1 Antibody [NBP2-47125] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse testes using FAM8A1 antibody at 1:500 dilution. The antibody generated a cytoplasmic staining with intense brown dots (potentially the staining of the ER) in each of the primary and secondary spermatocytic cells.