FluoroFinder

	Supplier	Novus Biologicals, a Bio-Techne brand
	Catalog #:	NBP2-22533 (View supplier product page)
	Size	200 ug
	Price	$399.00
	Antigen	CXCL11
	Clone	10C6
	Host	Mouse
	Isotype	IgG1
	Conjugate	Unconjugated
	Target Species	Human
	Applications	WB, ELISA
	Description	Mouse CXCL11/I-TAC antibody [Purified] reacts with Human

	CXCL11	Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC. This antimicrobial gene is a CXC member of the chemokine superfamily. Its encoded protein induces a chemotactic response in activated T-cells and is the dominant ligand for CXC receptor-3. The gene encoding this protein contains 4 exons and at least three polyadenylation signals which might reflect cell-specific regulation of expression. IFN-gamma is a potent inducer of transcription of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

		Western Blot: CXCL11/I-TAC Antibody (10C6) [NBP2-22533] - Analysis of 0.25 ug of recombinant human ITAC and induced HeLa cell culture supernatant onto a 4-20% Tris-HCl polyacrylamide gel.
		ELISA: CXCL11/I-TAC Antibody (10C6) [NBP2-22533] - Sandwich ELISA of Human ITAC was performed using the Thermo Scientific ELISA Kit by coating a blank 96-well microtiter plate with 100ul per well of Human ITAC moncolonal antibody in duplicate at 1, 3, 4, 5, 7, and 9ug/ml in DPBS and incubating for 12-18 hours at 4C. The plate was aspirated and blocked with 300ul per well of 4% BSA and 5% sucrose in DPBS for 1 hour at room temperature. Human ITAC recombinant protein at 50ul per well was added in duplicate at 4000, 1600, 640, 256, 102.4, 40.96, and 0 pg/ml for 2 hours at room temperature along with 50ul of Human ITAC biotinylated monoclonal antibody in all applicable wells at 0.2ug/ml for 2 hours at room temperature. The plate was washed with ELISA Wash Buffer and incubated with 100ul per well of Streptavidin-HRP in all test wells at 1:80,000 dilution for 1 hour at room temperature and then washed and incubated with 100ul per well of TMB substrate for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.