FluoroFinder

	Supplier	Novus Biologicals, a Bio-Techne brand
	Catalog #:	NBP1-89095 (View supplier product page)
	Size	0.1 ml
	Price	$519.00
	Antigen	Cdc27
	Clone
	Host	Rabbit
	Isotype	IgG
	Conjugate	Unconjugated
	Target Species	Human
	Applications	WB, IHC, ICC/IF, IHC-P
	Description	Specificity of human Cdc27 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

	Cdc27	The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014]

		Immunocytochemistry/Immunofluorescence: Cdc27 Antibody [NBP1-89095] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
		Western Blot: Cdc27 Antibody [NBP1-89095] - Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
		Immunohistochemistry-Paraffin: Cdc27 Antibody [NBP1-89095] - Staining of human bronchus shows strong nuclear positivity in respiratory epithelial cells.