FluoroFinder

	Supplier	R&D Systems
	Catalog #:	AF6737-SP (View supplier product page)
	Size	25 μg
	Price	$149.00
	Antigen	ADNP
	Clone
	Host	Goat
	Isotype	IgG
	Conjugate	Unconjugated
	Target Species	Human
	Applications	WB, IHC
	Description	Human ADNP Antibody

	ADNP	Vasoactive intestinal peptide is a neuroprotective factor that has a stimulatory effect on the growth of some tumor cells and an inhibitory effect on others. This gene encodes a protein that is upregulated by vasoactive intestinal peptide and may be involved in its stimulatory effect on certain tumor cells. The encoded protein contains one homeobox and nine zinc finger domains, suggesting that it functions as a transcription factor. This gene is also upregulated in normal proliferative tissues. Finally, the encoded protein may increase the viability of certain cell types through modulation of p53 activity. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]

		ADNP in Human Brain. ADNP was detected in immersion fixed paraffin-embedded sections of human brain (inferior colliculus) using Goat Anti-Human ADNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6737) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
		Detection of Human ADNP by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ADNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6737) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ADNP at approximately 124 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.