53BP1 Specificity is Shown by Immunocytochemistry in Knockout Cell Line. 53BP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in 53BP1 knockout (KO) HeLa Human Cell Line cell line using Rabbit Anti-Human 53BP1 Monoclonal Antibody (Catalog # MAB18772) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

53BP1 in HeLa Human Cell Line. 53BP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human 53BP1 Monoclonal Antibody (Catalog # MAB18772) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human 53BP1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line and ZR-75 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human 53BP1 Monoclonal Antibody (Catalog # MAB18772) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for 53BP1 at approximately 350 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

53BP1 in Human Cervical Cancer Tissue. 53BP1 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Rabbit Anti-Human 53BP1 Monoclonal Antibody (Catalog # MAB18772) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western Blot Shows Human 53BP1 Specificty Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 53BP1 knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human 53BP1 Monoclonal Antibody (Catalog # MAB18772) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for 53BP1 at approximately 350 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.