CaMKII alpha/beta Monoclonal / FITC / 22B1

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NB110-96869F (View supplier product page)
Size 0.1 ml
Price $549.00
Antigen CaMKII alpha/beta
Clone 22B1
Host Mouse
Isotype IgG1
Conjugate FITC
Target Species Human, Mouse, Rat
Applications ELISA, IHC, ICC/IF, IHC-P
Description This antibody is specific for a and B subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in B).
About CaMKII alpha/beta and FITC
CaMKII alpha/beta The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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FITC Excitation and Emission Spectra