Vitronectin Polyclonal / Unconjugated /
Vitronectin Polyclonal / Unconjugated /
Product Details
| Supplier | R&D Systems | |
|---|---|---|
| Catalog #: | AF2349-SP (View supplier product page) | |
| Size | 25 μg | |
| Price | $149.00 | |
| Antigen | Vitronectin | |
| Clone | ||
| Host | Goat | |
| Isotype | IgG | |
| Conjugate | Unconjugated | |
| Target Species | Human | |
| Applications | WB, IHC | |
| Description | Human Vitronectin Antibody |
About Vitronectin and Purified
| Vitronectin | The protein encoded by this gene functions in part as an adhesive glycoprotein. Differential expression of this protein can promote either cell adhesion or migration as it links cells to the extracellular matrix through a variety of ligands. These ligands include integrins, plasminogen activator inhibitor-1, and urokinase plasminogen activator receptor. This secreted protein can be present in the plasma as a monomer or dimer and forms a multimer in the extracellular matrix of several tissues. This protein also inhibits the membrane-damaging effect of the terminal cytolytic complement pathway and binds to several serpin serine protease inhibitors. This protein can also promote extracellular matrix degradation and thus plays a role in tumorigenesis. It is involved in a variety of other biological processes such as the regulation of the coagulation pathway, wound healing, and tissue remodeling. The heparin-binding domain of this protein give it anti-microbial properties. It is also a lipid binding protein that forms a principal component of high density lipoprotein. [provided by RefSeq, Aug 2020] |
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Images
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Vitronectin in Human Bladder. Vitronectin was detected in immersion fixed paraffin-embedded sections of human bladder using Goat Anti-Human Vitronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2349) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular matrix. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
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Detection of Human Vitronectin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Vitronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2349) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Vitronectin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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Detection of Human Vitronectin by Simple WesternTM. Simple Western lane view shows human serum, loaded at a 1:300 dilution. A specific band was detected for Vitronectin at approximately 76 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Vitronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2349) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
Citations
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