Cell Adhesion Mediated by Siglec-5 and Neutralization by Human Siglec-5/14 Antibody. Recombinant Human Siglec-5 Fc Chimera (Catalog # 1072-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec-5 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Siglec-5/14 Mono-clonal Antibody (Catalog # MAB10721). The ND₅₀ is typically 0.04-0.2 µg/mL.

Detection of Siglec-5/14 in Human Monocytes by Flow Cytometry. Human blood derived monocytes were stained with Mouse Anti-Human Siglec-5/14 Monoclonal Antibody (Catalog # MAB10721, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂ Secondary Antibody (Catalog # F0102B).