FluoroFinder

	Supplier	R&D Systems
	Catalog #:	IC9125G-025 (View supplier product page)
	Size	25 Tests
	Price	$209.00
	Antigen	ROR gamma
	Clone	1181A
	Host	Rabbit
	Isotype	IgG
	Conjugate	Alexa Fluor 488
	Target Species	Human, Mouse
	Applications	FC, ICFC
	Description	Human/Mouse ROR gamma t/RORC2/NR1F3 Alexa Fluor® 488-conjugated Antibody Intracellular Flow Cytometry = ICFC

	ROR gamma	The protein encoded by this gene is a DNA-binding transcription factor and is a member of the NR1 subfamily of nuclear hormone receptors. The specific functions of this protein are not known; however, studies of a similar gene in mice have shown that this gene may be essential for lymphoid organogenesis and may play an important regulatory role in thymopoiesis. In addition, studies in mice suggest that the protein encoded by this gene may inhibit the expression of Fas ligand and IL2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
	Alexa Fluor 488	Alexa Fluor™ 488 (AF488, Alexa 488) has an excitation peak at 488 nm and an emission peak at 496 nm, and is considered a high-performance alternative to FITC. Alexa 488 is one of the most popular Alexa Fluor™ dyes and is widely used in Fluorescence Microscopy, flow cytometry, and for staining low expression markers. It is bright, highly photostable, resistant to pH changes, and less susceptible to photobleaching. Alexa 488 and is similar in size, brightness and application to DyLight™ 488, iFluor® 488 and CF®488A.

		Detection of ROR gamma t/RORC2/NR1F3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Rabbit Anti-Human/Mouse ROR gamma t/RORC2/NR1F3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC9125G) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC1051G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
		Detection of ROR gamma t/RORC2/NR1F3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Rabbit Anti-Human/Mouse ROR gamma t/RORC2/NR1F3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC9125G) and Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A). Quadrant markers were set based on control antibody staining (Catalog # IC1051G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.