Detection of PD-L1/B7-H1 in MDA-MB-231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast adenocarcinoma cell line was stained with Mouse Anti-Human PD-L1/B7-H1 APC-conjugated Monoclonal Antibody (Catalog # FAB1561A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). Adherent cells were prepared by either manual scraping or with TrypLE Express treatment with similar results. View our protocol for Staining Membrane-associated Proteins.

Detection of B7-H1/PD-L1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) (A) resting or (B) treated with 1 µg/mL LPS overnight were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and Mouse Anti-Human B7-H1/PD-L1 APC-conjugated Monoclonal Antibody (Catalog # FAB1561A). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.