FluoroFinder

	Supplier	R&D Systems
	Catalog #:	MAB2417-SP (View supplier product page)
	Size	25 μg
	Price	$159.00
	Antigen	Olig1
	Clone	257219
	Host	Mouse
	Isotype	IgG2b
	Conjugate	Unconjugated
	Target Species	Human, Mouse
	Applications	WB, IHC, IHC-P, IHC-Fr
	Description	Human Olig1 Antibody

	Olig1	Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in neuron differentiation and regulation of transcription by RNA polymerase II. Predicted to act upstream of or within neuron fate commitment. Predicted to be part of chromatin. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Apr 2022]

		Olig1 in Human Glioma. Olig1 was detected in immersion fixed paraffin-embedded sections of human glioma using 8 µg/mL Human Olig1 Monoclonal Antibody (Catalog # MAB2417) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
		Detection of Human Olig1 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line and SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human Olig1 Monoclonal Antibody (Catalog # MAB2417) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Olig1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.