FluoroFinder

	Supplier	R&D Systems
	Catalog #:	AF917 (View supplier product page)
	Size	100 μg
	Price	$489.00
	Antigen	MMP-12
	Clone
	Host	Goat
	Isotype	IgG
	Conjugate	Unconjugated
	Target Species	Human, Rabbit
	Applications	WB, IHC, IP
	Description	Human MMP-12 Antibody

	MMP-12	This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. This gene may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016]

		Detection of Human Pro-MMP-12 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human MMP-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF917) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Pro-MMP-12 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
		MMP-12 in Human Squamous Cell Carcinoma. MMP-12 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Goat Anti-Human MMP-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF917) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.